Other

Part:BBa_J100306:Design

Designed by: Monica Prudencio   Group: Campbell M Lab   (2016-10-08)


repClone Red (J100205) with wt TetR promoter (R0040)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 9
    Illegal AgeI site found at 2213
    Illegal AgeI site found at 2325
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

This part can be constructed by ligating the TetR promoter into repClone (J100205) following BsaI digestion.

References